The board-end 8x2 header on SCORPIO uses a different pin assignment than the regular Feather RP2040. No cutting, no jumpers, these pins have one job:
int8_t pins[8] = { 16, 17, 18, 19, 20, 21, 22, 23 }; Adafruit_NeoPXL8 leds(NUM_LED, pins, NEO_GRB);
Other than this pin list change, most of what’s previously stated on the RP2040 page applies. More informative though, the SCORPIO board has its own dedicated guide!